The Genomics Core provides UT faculty, staff, and students access to state-of-the-art equipment and training. As a shared user facility, we provide access to high throughput sequencing and associated laboratory equipment, Sanger sequencing via mail-in options, and DNA sequence analysis support to the UT research community.
The UT Genomics Core provides high-throughput next-generation sequencing services to all interested on-campus and off-campus researchers. Sequencing is performed using the Illumina NovaSeq and MiSeq instruments. We recommend consultation with Genomics Core staff prior to project initiation. While we strive for successful sequencing every time, we cannot guarantee project success, and in all cases, the user will be billed for cost and labor for Genomics Core effort as per our fee structure. All samples are assumed to be safe and no greater than BSL1, unless otherwise agreed upon in advance.
Services
- DNA Extraction
- Genome and Metagenome Library Preparation
- Metabarcoding of Amplicon Products
- RNA Seq
- Illumina MiSeq Sequencing
- Illumina NovaSeq Sequencing
Project Workflow
- DNA/RNA extraction
- Nucleic acid is extracted and purified
- Library Prep
- Prepare sample for sequencing by adding appropriate adapters
- Sequencing
- Read the sequence of the sample and create raw data
- Analysis
- 3 Steps: Primary and secondary analysis, and interpretation
Contact us at genomicscore@utk.edu.
DNA Extraction
Some DNA extractions are suitable for automation, and some are better suited for manual extraction. Automated extractions are performed on a KingFisher Flex using Zymo Research kits and manual extractions are performed using Qiagen extraction kits. Please contact Core Facility staff to discuss which method is best for your sample type.
Internal UT User |
External Academic |
External Non-Academic |
|
|---|---|---|---|
Manual DNA extraction, each |
$25 | $32 | $39 |
Automated DNA extraction, 1-48 samples, each |
$15 | $19 | $23 |
Automated DNA extraction, 49-96 samples, each |
$12 | $15 | $19 |
User rates subject to change without notice or as material costs change. Troubleshooting may necessitate additional costs.
Genome and Metagenome Library Preparation
Genome and metagenome libraries are prepared using Illumina’s DNA Prep kit (formerly Nextera DNA Flex Library Prep). The starting material needed is 50-250 ng of DNA in less than 15 uL volume, diluted in Tris-HCl, pH 8. The DNA should have an absorbance of 260/280 in the 1.8-2 range and 260-230 in the 2-2.2 range. Contact Core Facility staff if your template DNA does not meet these specifications.
The costs below are for library construction of extracted, quantified DNA. Next you will need to select the appropriate MiSeq or NovaSeq run.
Internal UT User |
External Academic |
External Non-Academic |
|
|---|---|---|---|
Genome and metagenome preps, 1-11 samples, each |
$65 | $82 | $100 |
Genome and metagenome preps, 12-48 samples, each |
$53 | $67 | $82 |
Genome and metagenome preps, >48 samples, each |
$42 | $53 | $65 |
User rates subject to change without notice or as material costs change. Troubleshooting may necessitate additional costs.
Metabarcoding of Amplicon Products
Amplicon libraries are prepared with Illumina’s two-step PCR Nextera XT amplicon protocol. Users can prepare their own PCR products or Core staff can fully prepare the amplicon libraries with common primers for 16S, ITS, and COI. Please contact Core staff prior to preparing your own library to confirm compatibility with Illumina sequencing platforms, as specific adapters are required. These are the costs for library construction of extracted, quantified DNA. For full library prep, DNA should be diluted to 10 ng/ul, with 10 ul provided. Next you will need to select the appropriate MiSeq or NovaSeq run.
Internal UT User |
External Academic |
External Non-Academic |
|
|---|---|---|---|
Full Amplicon library prep, up to 24 samples |
$1,050 | $1,325 | $1,625 |
Full Amplicon library prep, 25-48 samples |
$1,300 | $1,625 | $2,000 |
Full Amplicon library prep, 49-96 samples |
$1,750 | $2,200 | $2,700 |
Amplicon library prep of post-PCR product, up to 24 samples |
$800 | $1,000 | $1,225 |
Amplicon library prep of post-PCR product, 25-48 samples |
$1,000 | $1,250 | $1,550 |
Amplicon library prep of post-PCR product, 49-96 samples |
$1,375 | $1,725 | $2,100 |
User rates subject to change without notice or as material costs change. Troubleshooting may necessitate additional costs.
RNA Seq
RNA libraries are prepared using Zymo Research’s Zymo-Seq RiboFree Total RNA Library Kit, which can include an rRNA depletion step. The minimum input amount of RNA is 100 ng, but the recommended input is 500 ng. RNA should have A260/A280 and A260/A230 ratios >1.8 and be DNA-free. Please check that this kit is compatible with your needs.
The costs below are for library construction of extracted, quantified and quality confirmed RNA. Next you will need to select the appropriate MiSeq or NovaSeq run.
Internal UT User |
External Academic |
External Non-Academic |
|
RNA prep of up to 12 samples, each |
$105 | $132 | $161 |
RNA prep of 13-48 samples, each |
$95 | $119 | $146 |
RNA prep of >48 samples, each |
$85 | $107 | $130 |
User rates subject to change without notice or as material costs change. Troubleshooting may necessitate additional costs.
Illumina MiSeq Sequencing
The Illumina MiSeq is a benchtop sequencer specialized for lower-output runs on single-lane flow cells, offering Illumina’s longest read length of up to 300 nucleotides, paired-end. The MiSeq is ideal for amplicon sequencing, such as for bacterial 16S rRNA, and small genomes. It can also be used to test larger runs prior to running them on the larger Illumina NovaSeq.
The appropriate Illumina MiSeq flow cell for your project is determined by the depth of coverage needed, as well as the desired read length. Contact Core facility staff for advice on which flow cell is right for your project. The costs below are for a PREPARED library. Costs for library prep done at the Core facility listed on this website are in addition to the cost of the flow cell.
Internal UT User |
External Academic |
External Non-Academic |
|
v2, 50 cycle flow cell, 2 x 25 nt Sequencing, ~12-14 million read pairs |
$1,380 | $1,585 | $1,735 |
v2, 300 cycle flow cell, 2 x 150 nt Sequencing, ~12-14 million read pairs |
$1,685 | $1,905 | $2,055 |
v2, 500 cycle flow cell, 2 x 250 nt Sequencing, ~12-14 million read pairs |
$1,850 | $2,085 | $2,235 |
v3, 150 cycle flow cell, 2 x 75 nt Sequencing, ~22-25 million read pairs |
$1,500 | $1,710 | $1,860 |
v3, 600 cycle flow cell, 2 x 300 nt Sequencing, ~22-25 million read pairs |
$2,320 | $2,580 | $2,730 |
Micro cycle flow cell, 2 x 150 nt Sequencing, ~4 million read pairs |
$880 | $1,060 | $1,210 |
Nano v2, 300 cycle flow cell, 2 x 150 nt Sequencing, ~1 million read pairs |
$685 | $855 | $1,005 |
Nano v2, 500 cycle flow cell, 2 x 250 nt Sequencing, ~1 million read pairs |
$765 | $935 | $1,085 |
MiSeq run only |
$300 | $450 | $600 |
User rates subject to change without notice or as material costs change. Troubleshooting may necessitate additional costs.
Illumina NovaSeq Sequencing
The NovaSeq 6000 is a large-scale sequencer from Illumina, generating up to 10 billion read pairs in less than two days using patterned flow cell technology, with scalable through-puts to accommodate most projects. Careful consideration of sample numbers and flow cell output is crucial for effective use of this platform. The high yields of the NovaSeq flow cells may present difficulties in efficiently filling flow cells for many researchers. As such, we offer some flow cells by individual lanes to reduce costs for users. Contact Core Facility staff to discuss lane sharing options. Custom read lengths are also available, generally requiring whole flow cell reads.
The appropriate Illumina NovaSeq flow cell for your project is determined by the depth of coverage needed, as well as the desired read length. Contact Core facility staff for advice on which flow cell is right for your project. The costs below are for a PREPARED library. Costs for library prep done at the Core facility listed on this website are in addition to the cost of the flow cell.
For assistance calculating coverage necessary for your specific project, visit Illumina’s Coverage Calculator.
Internal UT User |
External Academic |
External Non-Academic |
|
SP 200 cycle flow cell, 2 x 100 nt Sequencing, ~650-800 million read pairs |
$3,720 | $4,135 | $4,385 |
SP 300 cycle flow cell, 2 x 150 nt Sequencing, ~650-800 million read pairs |
$4,015 | $4,445 | $4,695 |
SP 500 cycle flow cell, 2 x 250 nt Sequencing, ~650-800 million read pairs |
$5,415 | $5,925 | $6,175 |
S1 200 cycle flow cell, 2 x 100 nt Sequencing, ~1.3-1.6 billion read pairs |
$6,175 | $6,725 | $6,975 |
S1 300 cycle flow cell, 2 x 150 nt Sequencing, ~1.3-1.6 billion read pairs |
$6,645 | $7,215 | $7,465 |
S2 200 cycle flow cell, 2 x 100 nt Sequencing, ~3.3-4.1 billion read pairs |
$11,035 | $11,835 | $12,085 |
S2 300 cycle flow cell, 2 x 150 nt Sequencing, ~3.3-4.1 billion read pairs |
$11,735 | $12,575 | $12,825 |
S4 200 cycle flow cell, 2 x 100 nt Sequencing, ~8-10 billion read pairs |
$15,630 | $16,670 | $16,920 |
S4 300 cycle flow cell, 2 x 150 nt Sequencing, ~8-10 billion read pairs |
$17,355 | $18,485 | $18,735 |
NovaSeq run only |
$500 | $750 | $1,000 |
User rates subject to change without notice or as material costs change. Troubleshooting may necessitate additional costs.
The Genomics Core houses additional equipment available for use.
- Pippen Prep
- The Pippin Prep performs automated gel extraction. and is available for both assisted and unassisted use. Contact Core staff for information on available cassettes.
- Bioruptor
- The Bioruptor uses ultrasound to fragment DNA and is available for both assisted and unassisted use.
- Bioanalzyer
- The Agilent Bioanalzyer quantifies and qualifies DNA and RNA. The appropriate chip is determined by the expected product size and concentration. Chips hold either 11 or 12 samples.
Internal UT User |
External Academic |
External Non-Academic |
|
|---|---|---|---|
Pippin Prep, unassisted (a) |
$10* | $16* | $20* |
Pippin Prep, assisted (b) |
$45* | $69* | $90* |
Pippin Prep, cassettes |
$60 | $60 | $60 |
Bioruptor, unassisted (a) |
$10 | $16 | $20 |
Bioruptor, assisted (b) |
$20 | $31 | $40 |
Bioanalyzer, High Sensitivity chips, 11 samples max |
$75 | $115 | $150 |
Bioanalyzer, standard sensitivity chips, 12 samples max |
$60 | $92 | $120 |
Labor, Manager |
$45 | $69 | $90 |
(b) Assisted use implies that the user has not received instrument training and will require assistance from Core facility personnel for sample analysis
*Plus $55 for cassette costs, if not provided by the user
User rates subject to change without notice or as material costs change. Troubleshooting may necessitate additional costs.
The UTGC no longer offers Sanger Sequencing in house. Sanger Sequencing must be sent off campus. We offer drop boxes across campus for those researchers wishing to mail in their samples for Sanger sequencing. Samples are mailed overnight to Eurofins Genomics with results returned by, or before, noon the next day. The quality of your results is dependent upon the quality of DNA that you submit to Eurofins, so it is critical that you follow their directions in sample preparation and sample labeling (request free bar codes).
Pricing
The current price for Eurofins Sanger sequencing is $4.00/tube or $3.00/well for 96-well plates, as well as $1.00/sample for Load Only samples. Eurofins offers >70 universal sequencing primers for free with your sequencing order. Shipping is also free. Results (.seq and .ab1 files) will be returned the next day before noon, including on Saturday.
*Eurofins offers a variety of other sequencing options. Please download their price sheet for more information.
**Eurofins offers a Power Read service for an additional $2.50/tube for difficult-to-sequence samples (i.e., templates with G/C-rich content, difficult secondary structures, homopolymeric sequences, tandem repeat stretches, bisulfite-treated DNA, shRNA, etc.).
Samples can be dropped off for free shipping on the Ag campus at this location:
- The UTIA Omics Hub Lab, Plant Biotechnology Building, Room 308 (contact info: Sujata Agarwal; sagarwal@utk.edu; 865-974-0676)
Some individual orders qualify for free shipping. See details here.
Veronica BrownLab Manager 865-974-8031 |
Andrea TrentResearch Technician 865-974-8031 |
Additional Resources
Existing users may place orders via Stratocore. New users contact Genomics Core staff.
For information on how large sequencing data files are transferred, please see the High Performance and Scientific Computing Genomics and Sequencing Support page or contact OIT_HPSC_Genomics@utk.edu for more information.